Apis dorsata Binghami is one of the endemic species of Sulawesi, Indonesia. Apis dorsata Binghami has not yet been cultured, but produces more honey and is richer in secondary metabolites than Apis mellifera. Empirically, the people of Minahasa, North Sulawesi, Indonesia have long been using honey bee nest as medicine. Research has been conducted to determine the antihyperlipidemic activity of infusion extract of Apis dorsata Binghami nest. Apis dorsata Binghami nest has obtained from natural forests, Minahasa, North Sulawesi, Indonesia. Extraction was done by the infusion method. Antihyperlipidemic analysis using pathogenic free white mice. Blood lipid profiles measured were total cholesterol, low-density lipoprotein (LDL) and triglycerides. This study used a completely randomized design with five treatments and three replications. Data from the study were analyzed variance. The results of this research showed that the treatment of 5% and 10% infusion extract Apis dorsata Binghami nest, significantly reduce total cholesterol, LDL and rat blood triglycerides. Further research on the antihyperlipidemic activity of Apis dorsata Binghami nest extract has the potential to produce antihyperlipidemic herbal drugs.