Drug resistance due to the extensive mishandling and over exploitation of these antibiotics has become a serious problem. In order to reduce the burden of these antibiotics, we worked on extracts from medicinal plant products and determined its antimicrobial activity against specific bacterial strains. In this study, our objective is to determine the antimicrobial potential of leaf extracts of guava (Psidium guajava) against three bacterial strains i.e. Bacillus subtilis, Salmonella enteritidis and Pseudomonas aeruginosa. For these studies four different solvents of increasing polarities (petroleum ether, hexane, methanol, and aqueous i.e. distilled water) were used for extraction and determined its phytochemical analysis and also measuring its antimicrobial activity using disc-diffusion method. The results of these studies showed that methanol and aqueous extract of Psidium guajava leaves showed inhibitory activity against Bacillus subtilis, Salmonella enteritidis and Pseudomonas aeruginosa. Overall, guava leaf-extract considered as one of the best candidate for natural antimicrobial agent.