In this present work we for the first time developed an efficient and stable micropropagation protocol ofCanavalia gladiata (Jacq) DC. an underutilised or lesser known legume via cotyledonary node explant derived from in vitro seedlings. Highest (73.3 %) percentage of seed germination was found using fresh seeds on half-Murashige and Skoog’s (MS) medium without any plant growth regulators at 6-10 d of culture. MS + BAP 1.0 mg l-1 was found to be most successful for induction and proliferation of multiple shoots. In vitro shoot multiplication percentage was found to be 100 % with highest c.a. 8.7 shoots per explant by an average 4.8 cm shoot length on the above medium. In vitro nodal segments harvested from the first shoots of original cotyledonary node were used as explant source for further shoot multiplication. This facilitated the production of average 128 (8 shoots/CN x 4 in vitro nodes/shoot x 4 shoots/in vitro nodes) number of shoots per in vitro cotyledonary node after the 1st harvest at 30 d, whereas a total c.a. 224 shoots (8x4x4 1st + 4x4x4 2nd + 2x4x4 3rd) were harvested after 3rd culture of the mother cotyledonary node with two whole cotyledons at 75 d. Cent percent rooting was observed in in vitro derived shoots on ½ MS+ 0.5 mg l-1 IAA showed 4.2 no of roots per shoot with 5.8 cm root length. Plantlets with well-developed roots were acclimatized in sand and soil (1:1) and placed in pot with normal soil.