Phytochemical screening using the standard qualitative technique revealed the presence of phenols, terpenoids, tannins, glycosides and anthraquinones in the leaves extract and saponins, alkaloids, phenols, steroids, tannins, flavonoids, anthraquinones, glycosides and sugar in the bark extract. Paper disc diffusion method was applied in evaluating the antibacterial activity of the crude extract against Staphylococcus aureus and Escherichia coli using the disc diffusion method. The bark extract was active against S. aureus and E. coli measuring clear zones of inhibition of 21mm and 22.66mm respectively. This indicate the presence of these bioactive components in S. lineatum, which proves its potency in the treatment of some bacterial infections.