Adansonia digitata L (Baobab) is a majestic, a deciduous and a large iconic tree revered in Africa for its nutritional and medicinal value. The plant parts are used to treat many diseases such as fever, diarrhea, dysentery, inflammation, asthma, malaria, anemia and microbial ailments. However, there is very little data on active ingredients in fruits, leaves, bark-stem and roots responsible for its biological properties. The aim of the present study was to quantify plant phenolic constituents and to evaluate antioxidant properties of different parts of Adansonia digitata. The LC-MS/QTOF experiment led to the characterization of 21 constituents including, rutin, fumaric acid, quercetin-3-β-d-glucoside, 4-hydroxybenzoic acid, protocatechuic acid, catechin, gentisic acid, vanillic acid and scutellarin. The flavanol catechin was found to be the most predominant phenolic constituent present in the four plant parts (0.0165-0.0233 mg/g of dry plant material). The highest amount of phenolic present was rutin (0.392 mg/g of dry plant material) in leaves extract. In DPPH radical scavenging assay, the effect on reducing free radicals increased in a dose dependent manner. On overall, among the four parts extracts, leaves extract showed highest antioxidant activity, with inhibition percentage ranging from 22.19% to 72.15%. In summary, findings reported that Adansonia digitata could be a natural source of phenolic constituents with antioxidant activity.