Samangadi churna is commonly used Ayurveda formulation having 8 herbs. Samangadi churna is used to enhance Medha (memory), Aayu(Longevity), and Bala (Muscle strength). Present study is designed to done quality control of Samangadi churna using identifing physiochemical parameter and discover a simple, sensitive and accurate HPTLC method for fingerprinting as well as detection and quantification of active marker phytoconstituent gallic acid and plumbagin in Samangadi churna for standardization. Physicochemical Parameter of Samangadi Churna reported using method given Ayurvedic Pharmacoepia of India. Methanolic extraction was performed for HPTLC analysis of churna. HPTLC was for gallic acid using mobile phase of Toulene: Chloroform:Methanol(4:4:1 v/v/v) and detection at 366 nm. For identify plumbagin using mobile phase containing Toluene: Ethyl acetate: Formic acid (7:3:0.1 v/v/v). The Rf was detected for gallic acid at 0.42 and plumbagin at 0.7 in Samangadi churna. Gallic acid was detected to be 120.8 µg in 150 mg of Samangadi churna sample while Plumbagin was detected to be 34.61 µg in 100mg Samangadi churna sample. The HPTLC method was developed for determination of gallic acid and plumbagin in Samangadi churna. The proposed HPTLC method and physiochemical parameters value can be used for the routine quality control of Samangadi churna.