Nyctanthes arbor-tristis is a small shrub tree belongs to Oleaceae family. It is a highly traditional medicinal plant known as Harsingar or Night Jasmine. Its native to India but distributed in Himalayan and Nepal region. It is found as ornamental plant in Indian garden and temple side. Ethnobotanical survey and informations about the plant, the five villages of Agra city nearby Yamuna bank followed by Runakta to Poiya Ghat were selected for observation. Ethnobotanical observations were made on plant of Nyctanthes arbor-tristis and major discussed by five villager rural people like Farmer, Vaidhya, Hakim and Medicine men of the district of Agra regarding, medicines, socio-religious beliefs and the material culture. Household survey was conducted on a random basis for obtain information on people's perceptions on conservation, amount of availability and uses of N. arbor-tristis. A structured interview method followed by set of questions was used for the study. The flowers leaves, fruits, bark and seed of this plant is extensively used in Ayurvedic medicine for the treatment of various diseases by local people of Agra region. Therefore, it should reproduced and promoted cultivation in rural as well in urban areas.