With the recent shift of medicines to phytotherapy, ethnobotany currently provides an important framework for drug development. Herein, an ethnobotanical study, field mapping, and physiological review were conducted on folkloric medicinal plants utilized by the Tausugs of Isabela City, Basilan Province. A snowball sampling was employed to 50 key informants through a semi-constructed survey questionnaire. Voucher specimens during field mapping were collected for scientific identification. Results revealed 39 species belonging to 27 Families that are utilized by the Tausugs of Isabela City, Basilan Province to cure various diseases. Family Fabaceae is the most represented with five (5) species. Common health problems being experienced by the Tausugs include fever, cough, wounds, physical relapse (locally referred to as bughat), hyperacidity, and abdominal pain. Leaves are the most exploited plant part through the decoction process. Physiological reviews validate the medicinal plant’s use and effectiveness, except for Musa acuminata bract which needs further studies.