The present study emphasizes on the isolation and molecular identification of endophytic fungi from the leaf, stem and flower of medicinal plant Maytenus emarginata (Wild.) Ding Hou. for the production of biologically important silver nanoparticles. Molecular characterization carried out by using 18s rRNA ITS universal primers and sequence data submitted to NCBI (Acc. No.MN794565). ITS sequencing of 18S rRNA region shows 100% homology with NCBI clade Alanphillipsia aloeigena isolate (NR137121), Biosynthesis of Silver nanoparticles was carried out using mycelium free filtrate incubated with 1mM aqueous solution of silver nitrate (AgNO₃). After observing the solution changes from colourless to brown colour indicates the bioreduction of silver ion to silver nanoparticles. The isolated endophyte is new report to Maytenus emarginata (Wild.) and it is good source of AgNPs.