Our present study was undertaken to identify the new active constituent from Mentha spicata for their antioxidant activities to determine novel active to improve the treatment of diseases. In these studies leaf extracts and callus extracts in various solvents (Ethanolic, DMSO). The antioxidant potential of the Mentha spicata was investigated by ferric ion reducing antioxidant power (FRAP assay). Among those studied, the ethanolic extract of M.spicata leaf showed the highest phenolic content with 0.0110 mg gallic acid /mg of dry weight which was followed by dry weight extract of callus. The total antioxidant activity of ethanolic leaf extract was higher when compared to dry weight extract of callus. The leaves of Mentha spicata have excellent antioxidant activities. Hence, it can be employed as a new active ingredient in the antioxidant formulation in plant species. There is no report on the antioxidant activity of Mentha spicata L.