Vitex negundo has been traditionally in use since time immemorial. The current study was done to analyze the phytochemicals present in the plant and also to evaluate its antimicrobial activity against Salmonella typhi. Leaves of Vitex negundo were grinded and were extracted using distilled water. Preliminary phytochemical screening of the extract confirmed the presence of alkaloid, flavonoids, proteins, amino acids, glycosides, tannins, carbohydrates, coumarins and saponins. The Protein concentration of the extract was found using Lowry’s method. The presence of these bioactive constituents is associated with the antimicrobial activity of the plant. By disc diffusion method, the bacterial pathogen Salmonella typhi was found to be susceptible in leaf extracts of the vitex negundo and showed good activity against the pathogen.