Introduction: Sandhana Kalpana is a distinctive dosage variety in which acidic and alcoholic fermented preparations are formulated. Under fermentation preparations liquid media like decoction, expressed juices etc., sweetening agents, additives, fermentation initiators like Woodfordia fruticosa Kurz. And Madhuca indica Gmel. Or sediment are bring together in an inert vessel and sealed for precise time duration to make the process of fermentations. To initiate or potentiate the fermentation process numbers of components are required which serve as natural carries of the fermenting organism. From these, fermentation initiator is one of the main drugs for fermentation and sediment of previous fermentation also can use, but precise quality of these flowers and sediment was ailing studied. Therefore this microbiological study carried out to know the role of these flowers, sediment and ancient perception behind it. Aim & objectives: To assess the role of Woodfordia fruticosa Kurz., Madhuca indica Gmel. Flowers and Sediment in fermentation process w.s.r. to their microbiological flora in Kharjurasava Material & Methods: Kharjurasava was prepared as per reference from Yogaratnakar by adding W. fruticosa flowers and another by adding M. indica flowers as fermentation initiator. Total aerobic microbial count of drugs in Kharjurasava and Sediment was carried out by serial dilution agar plate technique. Observation & Results: Kharjurasava prepared with W. fruticosa flowers and their Sediment have shown more number of colonies of yeasts, where Kharjurasava prepared with M. indica flowers and their Sediment have shown few colony forming units of yeasts. Conclusion: Microbiological assessment for yeast cells has shown that W. fruticosa and their Sediment has more number of yeast colonies than that of M. indica and their Sediment.