The present study was conducted to evaluate the current status, population density and availability of Dactylorhiza hatagirea in different microhabitat. This work also focused on the threat and conservation status. The population density of Dactylorhiza hatagirea was ranged from 0.2 - 0.9 ind/m². According to the microhabitats, the density of Dactylorhiza hatagirea was varied in the present study sites. In the open grass land habitats the highest recorded density was (0.9 ind/m²) whereas disturb/ waste land was contained least density (0.2 ind/m²). A total of 58 plant species belonging to 51 genera and 31 families were recorded from the different microhabitats. We found that, the least density of Dactylorhiza hatagirea, because of some major factors like over exploitation for mainly local uses and trade, unsustainable grazing and trampling as well as the higher density of weedy and invasive species in the present study area.