Plant tissue culture techniques provides an excellent plate form in modern medicinal plants studies. The in-vitro callus development in a controlled environmental and media conditions generates undifferentiated mass of cells of medicinally important plant that have the capability to generate and release essential Phyto-Pharmaceuticals when undergoes cell suspension culture studies. Such methodology is of utmost importance when the plant is endangered and at the erg of extinction, Gardenia gummifera Linn. f is one such herbal plant of varied medical importance but its existence is getting declined and its over exploitation made its presence much reduced. The present study is focused on optimization of callus and cell suspension culture of leaf ex-plant of Gardenia gummifera Linn. f and the application of selected elicitors (Salicylic acid) (SA) was done in different concentrations as 100, 300, 600 µM. The intracellular and extracellular harvesting of cell suspension culture for phytochemical was done for all different concentrations at 24 and 96 hours respectively. The quantitative analysis of Phenolic and Flavonoid components was done as per the standard spectrophotometric method in which gallic acid and Quercetin used as standards. The result suggests that the elicitor (SA) had the potential to increase the accumulation of phenolic and flavonoid content at a concentration of 300 µM post 24 hours application.