For improvement of different crop plants, mutation breeding is most reliable technique. Mutation breeding widely used for the improvement of plant characters and has been a powerful and effective tool in increasing variability especially for self-pollinated crops with narrow genetic base. Assessment of biological damage induced by different doses of physical and chemical mutagens is an important aspect during mutation breeding programmes. The current experiment was conducted to assess the biological damage induced by different doses/concentrations of gamma rays and ethylmethane sulphonate in two cultivars of faba bean, an underutilized crop viz., vikrant and PRT-12. Both single and combination treatments of gamma rays and EMS were used in this experiment. Biological damage through seed germination and percentage injury was calculated in both laboratory and field. A gradual decrease was noticed in seed germination in both B.O.D. petriplates experiment and in field with increasing doses of single and combined treatments of gamma rays and EMS in both the varieties (Vikrant and PRT-12) of faba bean. Variety Vikrant showed more reduction in seed germination than variety PRT-12. The reduction in seed germination was highest in gamma rays+EMS combined treatments followed by EMS and gamma rays.