Nanoparticles have potential effects in life sciences and pharmaceutical applications. Among the nanoparticles, the silver nanoparticles are playing a major role in the field of biomedical nanotechnology and nanomedicine. A green method to synthesize silver nanoparticles using the Cucumis melo fruit extract has been developed. Biosynthesis of silver nanoparticles using aqueous extract of Cucumis melo fruit was prepared and its characterization by using various techniques such as UV-Visble spectroscopy, Scanning electron microscopy (SEM), Energy dispersive x-ray spectroscopy (EDX), Particle size analyzer and X-ray diffraction (XRD) methods. In fruit-mediated synthesis, after addition of Cucumis melo fruit extract to the aqueous solution of AgNO3, the mixture showed a gradual change in color at room temperature from orange to dark brown and the color intensified after 24 hours. The synthesized nanoparticles observed at 320nm to 380nm in UV-Visible spectroscopy revealed the presence of AgNPs. Morphology evaluation by SEM analysis showed the spherical structure of nanoparticles and XRD shows the crystalline size. Particle size was found to 35nm. The current study revealed that silver nanoparticles can be synthesized in a biological method using Cucumis melo fruit extract. The nanoparticles play a pivotal therapeutic importance because of their ability to cross biological barriers and stimulate self-healing cell responses augmenting biocompatibility.