In 2018 and 2019, suspected phytoplasma symptoms of typical leaf chlorosis, witches' broom and small leaf were recorded on seventeen weed species in and around agricultural fields in various parts of India. Symptomatic weed species were tested for phytoplasma interaction using nested PCR assays using standardised phytoplasma-specific primary pairs, ‘P1 / P7 and R16F2n / R16R2’.Ten of the seventeen symptomatic weed species collected, viz. Positive to phytoplasma were “Digitaria ciliaris, Phalaris minor, Eleusine indica, Cynodon dactylon, Parthenium hysterophorus, Dichanthium annulatum, Cannabis sativa, Datura stramonium, Digitaria sanguinalis and Oplismenus burmanni”. BLAST and phylogenetic study of 16Sr-DNA phytoplasma sequences of ten positive weed species confirmed the relationship of three distinct phytoplasma types. 16Sr-I, 16Sr-II, 16Sr-XIV. Phytoplasma interaction with D. Ciliaris (16Sr-XIV), E. Indica (16Sr-XIV group) and P. minor (16Sr-I group) were new world phytoplasma records. The two weeds, though, viz. P. hysterophorus, O. burmanni. The new phytoplasma community (16Sr-II) in India identified burmanni hosts.