Holoptelea integrifolia L. belongs to family Urticaceae. Plants have a great potential to develop new drugs that would greatly benefit mankind and plant medicinal properties based on the existence of the specific physiologically active component contributing to the discovery for new biologically active therapeutic efficacy principles. The present study was carried out to evaluate the antimicrobial efficiency of methanolic and aqueous extract of leaves and young stem bark of Holoptelea integrifolia L. against the microorganisms Straphylococcus aureus bacteria and Penicillium notatum fungus. Aqueous and methanolic extracts concentrations (5%, 10%, 15% and 20%) were prepared from the leaves and young stem bark of Holoptelea integrifolia. The antimicrobial efficiency of the aqueous and methanolic extract concentration of each plant was tested using agar well diffusion method and zone of inhibition zone was measured in millimeters. Methanolic and aqueous extract of leaves and young stem bark showed positive results against Staphylococcus aureus from 5% to 20% concentration. 11mm zone of inhibition at 15% concentration for aqeous leaves extract, 11mm zone of inhibition at 15% and 20 % concentration for methanolic leaves extract, 8.5mm zone of inhibition at 5% concentration, 9.5mm at 10% concentration, 9.5mm zone of inhibition at 15%concentration and 11mm zone of inhibition at 20% concentration of young stem bark of aqeous extract. Methanolic Young stem bark extract showed negative results. Methanolic and aqeoues extract of leaves and young stem bark showed positive results against Penicillium notatum fungus from 5 % to 20 % concentration. The results obtained showed that the diameter of zone of inhibition increased with increase in concentration of extract and the antibacterial efficiency of aqeous and methanolic extracts of the plant was observed in the increasing order.