Brassica L. is one of the major agronomical valued genus with divergent oilseed crops and vegetables; belonging to the Brassicaceae family. The aim of the present analysis is to assess the pollen morphology of Brassica L. varieties viz. Tori-7, BARI Sarisha-6, BARI Sarisha-7, BARI Sarisha-10, BARI Sarisha-12 and BARI Sarisha-15 from Bangladesh by using light microscope. According to this analysis, the pollen grains of analyzed varieties are mostly prolate or subprolate, tricolpate, radially symmetrical with reticulate ornamented exine that furnishes an important source to get the systematic relationships of Brassica L.