Onion (Allium cepa L.) is an annual herb belongs to family Liliaceae. It is the most widely cultivated species of the genus Allium. In present investigation onion plants are evaluated for different growth and biochemical parameters like Chlorophyll content, Protein content, N, P and K content were evaluated. Evaluation of these parameters done using Onion plants with application of Arbuscular mycorrhizal (AM) fungi, Farm Yard Manure (FYM), Neem seed cake and combination of AM, FYM and neem seed cake etc. The research was conducted at the department of Botany, Nowrosjee Wadia College, Pune. The experimentation was done with three replicated randomized design for each treatment. Three control and three replicates were used for each set of experiment. The plastic pots (20 x20x 30 cm) of about 5 kg soil capacity were used for growing the Allium cepa L. seeds.