The native populations of Primula veris L. subsp. veris in Greece are often harvested directly from the wild due to known medicinal properties and ornamental potential and plant material is sold in national and international markets. Therefore, ex-situ conservation efforts are urgently needed for its sustainable use. The in vitro effect of gibberellic acid GA3 (three ways of application), light (16h photoperiod, 24h dark) and storage time (17, 14, 8 years, respectively, stored in 20 ^oC and relative humidity <5%) on seed germination of P. veris subsp. veris was investigated. MS basic medium was used enriched with 20 g/L sucrose and 6 g/L agar. After 60 days, the germination rate reached 50% when 250 mg/L GA₃ was added in the medium. Light did not affect seed germination ability; however, growth after germination was faster with high quality seedlings. No germination was detected in seeds stored for 17 and 14 years, while those stored for 8 years germinated at about 50%. The effect of GA₃ was critical, since the trials with no GA₃ presented <7% germination rate or no germination at all. Storing time of >10 years affected negatively the seed germination; therefore their renewal is necessary in seed banks at least every decade. The use of GA₃ favours the onset of germination. In the subsequent shoot proliferation stage, the MS medium enriched with either 4.4 or 6.6 μM BA in combination with 0.1 μM IBA and 0.3 μM GA₃ gave optimum results within a three week period in terms of shoot formation (100%), shoot number (3.2-3.7 shoots/ explant) and shoot proliferation rate (1.6-2.2) at the same time. All BA concentrations led to the production of multiple shoots to 100% of the explants, whereas no shoot formation was observed in the control treatment (BA-free). In the following rooting stage, IBA, irrespective of applied concentration (2.5 and 5 μM), exhibited 100% rooting and caused a 2.5-3-fold increase in root number (5.7-6.9 roots/rooted explant) compared to IBA-untreated explants. However, root length was greater (1.94 cm) in the case of the control, as both IBA concentrations had an inhibitory effect. The ex vitro acclimatization of rooted microplants was successfully completed (100% survival) within 26 days in the mist in a peat: perlite (1:1) mixture. Therefore, an efficient micropropagation protocol for P. veris subsp. veris was established from in vitro germinated seeds as starting material.