The largest and most widely distributed angiosperm is Legumes, which plays a significant role in the ecology of microbial community structure. Endophytic rhizobia have also been reported in the roots and stems of legumes and other plants. The present study aimed to isolate root nodule associated bacteria from five selected host legumes and to find out different harboring bacterial species in their root nodules. The highest bacterial diversity was observed in Mimosa pudica and Crotalaria albida. Totally 23 bacterial isolates from five different plant root nodules were isolated. Based on colony morphology and biochemical characters, isolates were identified as Agrobacterium sp (1), Bacillus spp (6), Bradyrhizobium spp (2), Pararhizobium spp (1), Enterobacter spp (3), Ensifer sp (1), Methylobacterium sp (1), Pseudomonas spp (3), Streptomyces sp (1), Rhizobium spp (3), Mesorhizobium sp (1). Of these 23 isolates, 13 belonged to nonrhizobial endosymbionts that come under Bacillus sp, Pseudomonas sp, Streptomyces sp, and Enterobacter sp. The remaining 10 where αProteobacteria that belong to the family of Bradyrhizobiaceae, Methylobacteriaceae, and Rhizobiaceae.