The intracellular signaling system based on calcium is linked to the external stimuli for mediating a wide range of endogenous responses. The concentration of the free cytosolic calcium ([Ca²⁺]_cyt) is central to this calcium-based signaling. In addition to ([Ca²⁺]_cyt), other components of this signaling cascade, such as calcium channels, calmodulin and protein kinase also play important role. Present work has reported the spatial localization of these components in mature pollen and receptive stage of stigma. This work is the first report of L-type calcium channels in the membrane of pollen as well as stigmatic papillae. Abundance of intracellular calmodulin in pollen and papillae has been reported. Protein kinase C has been found to be differentially distributed on the pollen coat and in the plasma membrane of papillae. These findings are evident of an active calcium-based signaling mechanism which could be prerequisite for success of initial phase of pollen stigma interaction.