Evaluate the functional quality of pollen aid appear in many approaches such as estimation the fertility of pollen or incompatibility, pollen vigor, pollen stigma interaction studies and for investigation of hybrid status. Main object of the work, to determine magnificent media for in vitro germination. Senna occidentalis L. (Fabaceae), Argemone mexicana L. (Papaveraceae) are wild plant. In Senna occidentalis L. after 60 min. highest germination and tube length reported are 89.38% and 73 % respectively in nectar of Euphorbia tithymaloides L., also superior result acquires in Brewbaker and Kwack (1963) media 88.56% germination and 71% tube length are reported and in Roberts et al. (1983) media 75% germination and 67% tube length are reported. Argemone mexicana L. (Papaveraceae) after 60 min. highest germination and tube length reported are 90.38% and 77% respectively in nectar Euphorbia tithymaloides L., also good result obtain in Brewbaker and Kwack (1963) media 92.55% germination and 76% tube length are reported and in Roberts et al.(1983) media 82% germination and 67% tube length are reported. In both species natural sugar of nectar performs as influencing factor.