Fusarium wilt is most important yield and growth-limiting factors of Pigeon pea (Cajanus cajan) cropping system. Further study was conducted in field in of wilted plot. These studies were repeated for two cropping seasons (2017-2018). Pigeon pea is an important pulse crop grown for its dhal and ranks sixth among legumes in area and production. It is a major source of protein to about 20 % of the world population and an abundant source of minerals and vitamins. The main constraints in boosting the yield of the crop are its susceptibility to diseases and insects. I am used two isolation tequenics for isolation of different types of Fusarium species in Pigeon pea infected plant samples and soil sample also. The Total fore fungal species was recovered from pigeon pea infected crop. The study result is maximum fungal species found in Varanasi district. I was notice that F. semitectum present in Varanasi but not recovered in Mirzapur and Fusarium equiseti also present in Varanasi but not recovered in Mirzapur district. But Fusarium udum recovered in all the sampling sights from Mirzapur district. F. monilyformy is present in Varanasi and Mirzapur sampling sights.