Phytoplasmas cause diseases in various weeds that can act as alternative natural hosts, facilitating the spread of phytoplasmas to other economically important plants and, therefore, increasing economic losses. Suspicious symptoms of phytoplasma of typical leaf chlorosis, witch's broom and small leaf were recorded in sixteen species of weeds in and around agricultural fields in different parts of Uttar Pradesh, India in 2017 to2019. to determine the association of phytoplasma through nested PCR assays using pairs of universal primers specific for phytoplasma, P1 / P7 and R16F2n / R16R2. Of sixteen species of symptomatic weeds collected, ten species of weeds, viz. Digitaria ciliaris, Phalaris minor, Eleusine indica, Cynodon dactylon, Parthenium hysterophorus, Dichanthium annulatum, Cannabis sativa, Amaranthus spinosus, Digitaria sanguinalis and Oplismenus burmanni have tested positive for phytoplasma. BLAST and phylogenetic analysis of 1.2 kb products of 16Sr DNA phytoplasma sequences from ten species of positive weeds confirmed the association of three different phytoplasmic groups, i.e. 16SrI, 16SrII and 16SrXIV. Association relationships of phytoplasma with D. ciliaris (16SrXIV group), E. indica (16SrXIV group) and P. minor (16SrI group) were the newest records of phytoplasma hosts in the world. However, the two weeds, viz. P. hysterophorus and O. burmanni have been reported as hosts for the new phytoplasma group (16SrII) in India. This article discussed detailed and updated information on occurrence, symptomatology, molecular characterization, transmission, taxonomy, genetic diversity and phytoplasma management approaches of weeds. Knowledge of the diversity of phytoplasmas will be expanded through recent studies and the availability of molecular tools for the identification of pathogens.