Pulses are one of the most important crops grown in India. Every year pulses are attacked by various insect and non-insect pests either in field or at storage. Chemical pesticides are very effective in controlling pests due to their quick mode of action and residual toxicity, but these chemical agents have serious clastogenic and cytotoxic effects on the crop as well. A descriptive field survey was conducted in two pulse growing districts of West Bengal, India to figure out the commonly used pesticides for pulse crop cultivation in these regions. From them three such pesticides Profenophos, Spinosad, Dimethoate were selected and germinated seeds of field pea (Pisum sativum var. arvense) were treated with them. Mitotic preparation were made following the standard aceto-orcein technique and assessed. The study revealed that the use of these chemical pesticides can reduce Mitotic Index (MI) and induce genotoxic effects showing chromosomal abnormalities.