A herbarium is a major source of systematic collection of dried and mounted plants specimens which are usually collected from natural habitat, mounted on sheets, and classify with their proper scientific information about the flora and described valuable significance in plant taxonomy which considered as a backbone of Botany. The present investigation was carried out on herbarium based taxonomic revision of Sida cordifolia L., Sida acuta (Burm f.), Sida cordata (Burm f.), and Sida rhombifolia L. were collected from different locality of Agra city. Multivariate analysis of specimens were also studied. The prepared specimens were identified and get relevant information from Regional Ayurveda Research Institute, Jhansi. This study aimed to provide proper taxonomic and biogeographical information on Sida species which will be useful for future study in teaching and botanical research.