In the present study, honey was used to as natural preservative in citrus fruit juice. Citrus limetta fruit juice samples were taken for study which is mostly consumed by the common people for their health issue. Different samples S1, S2, S3, S4, S5 and S6 of citrus fruit juice were made by adding honey in different concentration. Samples of citrus fruit juice stored at 4ºC for 15 days in the presence of honey were shown to inhibit opportunistic bacterial growth better compared to the sample stored without honey. Honey can be used in the citrus fruit juice for good taste, prevent the spoilage and boost an immune system. The following judgments suggest that organic honey can be used as an antibacterial agent as well as a preservative for Citrus fruit juice (Citrus limetta).