The microalgal species have the tendency to accumulate significant amounts of biomass which can be utilized for the production of wide range of biofuels, an alternative to fossil fuels. Microalgae are viewed as one of the potential feedstock for biomass production. The production of different biofuels depends on composition of biomass. In the present investigation, five microalgal isolates were identified using algal identification manuals. While Fourier transform infrared techniques were employed to analyze the functional groups in bio molecules. The microalgal isolates were identified as Scenedesmus sp., Chlorella sp., Monorapidium sp., Actodesmus sp. and Cyclidium sp. based on their surface morphology under microscope. Fourier infrared spectroscopy examination of algal biomass revealed the presence of –OH, –COOH, NH2, and C=O organic compound groups. The results suggest that the biomasses of all the isolates are very much sustainable for biofuel production.