Desmostachya bipinnata is a medicinal plant and contain many chemical constituents. Medicinal property of Plant is mostly affected by ‘Time (Kala)’ factor. As regards to time, it can be seasonal variation, lunar influence and diurnal variation. The concentration of chemical constituents in plants varies at different times of the day. For this work Desmostachya bipinnata plant was collected on the same time in morning (7:00 a.m.) as well as in evening (7:00 p.m.) during winter season. Extraction process of collected plant parts were carried out by Soxhlet apparatus. Then extract was analysed for qualitative and quantitative chemical test. This work shows the variation in concentration of flavonoids and phenol constituents. So, this work concluded that plant collection in morning time contain more flavonoids and phenol compound than plant collected in evening time.