The emergence of multi drug resistance has critically challenged the treatment of infectious diseases. It has thus created an urgent need for development of new antimicrobial agents as alternatives or adjuvants to the currently available antimicrobials. Phytochemicals are one such compound that are actively sought to be a safe alternative to antimicrobials. Hence, the current study was carried out to evaluate the antibacterial activity of Plectranthus amboinicus (Indian borage) against 30 multidrug resistant pathogens obtained from samples of urinary tract infections. For this purpose, the bioactive components were extracted from P. amboinicus using methanol, butanol, hexane, chloroform and petroleum ether. Qualitatively, methanol extract showed maximum antibacterial activity as compared to other solvents. The MBC of methanol extract against clinical isolates was determined to be in the range of 15-20mg/mL. The potential of methanol extract to inhibit the biofilm formation was also checked by tube method, which showed strong anti-biofilm activity against all 26 isolates that formed biofilms in test tubes. The phytochemical analysis of the aqueous extract showed presence of tannins, alkaloids, phenolic compound, terpenoids, carbohydrates, glycoside, flavonoids, triterpenoids and steroids indicating biochemically rich profile of the plant. Altogether, the observations of the present study suggested the potential use of P. amboinicus as an antibacterial agent or an adjuvant in similar preparations, for treatment of infections caused by multidrug resistant pathogens.