The Objective was to analysis the secondary metabolites of marine Streptomycetes MW9-1 (RW2-3), isolated from Bay of Bengal, Adirampattinam, Thanjavur district. The secondary metabolites were extracted by solvent extraction and Presences of active components are confirmed by GC-MS analysis. The identification of bioactive chemical compounds is based on the peak area, retention time, molecular weight and molecular formula. The GC-MS analysis of Streptomycetes MW9-1 (RW2-3) revealed the presence of molybdenum complex, Spiro compounds, iodo compounds, Chlorocompounds, Diethyl phthalate, Butyl phthalate, Phthalic acid mono ester, Phthalic acid diester, Acetyl benzoic acid were the major compounds present in fermented extracts of Streptomyces MW09-1 (RW2-3).