The word ‘Prakriti’ is the amalgamation of two words, ‘Pra’ and ‘Kriti’. The letter ‘Pra’ as suffix stands for pre or prior or before while the word ‘Kriti’ stands for ‘to make’ that means which is primary which proceeds what is made. ‘Prakriti’ is one amongst the major concepts of Ayurveda, which is adopted from Vedas, Puranas, Upanishads, and Darshanas. The insights of Vedas, Puranas, Upanishads, Darshanas and other preliminary literature of ancient India revealed that ‘Prakriti’ is Avyakta Tattva, an invisible phenomenon, the controller of entire universe, an equipoise of Trigunas (Sattva, Rajas, and Tamas) and all the living beings are originated from it. On the other hand, the concept of Prakriti has been delineated in a clinical perspective in Ayurveda, which suggests ‘Prakriti’ is Swabhava or nature or constitution of an individual, determined at the time of conception and remains unchanged throughout the life, any alterations in Prakriti cause imminent death. Every individual is a unique with their Prakriti. It distinguishes himself/herself anatomically, physiologically, psychologically and sociologically. The assessment of Prakriti is the foremost one in Dashavidha Parikshas (Ten-fold examination of a patient), which not only helps in the maintenance of health but also in the management of diseases. Keeping this in view, the present work has been taken to explore the different perspectives of concept of Prakriti dealt in Vedas, Upanishads, Darshanas and the treatises of Ayurveda in order to reveal the evolution of the Prakriti concept in ancient Indian literature.