In worldwide urinary tract infection is a second most common infection disease. Bacteria play on crucial role for UTI especially E. coli. In this present study to identify potential bioactive compounds from Cassia auriculata and their binding affinity was investigated against E. coli in computationally using Schrodinger suite. Based on the computational study, the following bioactive compounds 2, 4-Di-tert-butylphenol, 2H-Pyran-2-one, tetrahydro-6-tridecyl-, Diethyl phthalate, Palmitic acid Octadeca-9,12,15-trienoic acid, Pentadecanoic acid having better glide score and glide energy rather than other compounds. The compounds were taken into drug like evaluation by employing Qikprop. All compounds fall under desired ADME properties of six bioactive compounds are potential for further experimental analysis.