Gymnema sylvestre R. Br. (Family: Asclepiadaceae), is an important medicinal plant, rich in a group of oleanane triterpenoid saponins called Gymnemic acid, well known for its anti-diabetic activity. The vulnerability of the species emphasizes the need to exploit certain biotechnological strategy to maximize the production of its phytoconstituents. In recent times, developments in elicitation in plant tissue culture have opened new opportunities for the production of secondary metabolites. Thus, in the present study, elicitation for Gymnemic acid production in cell suspension culture was carried out using biotic elicitors viz. Yeast extract and Pectin. Cell suspension cultures were initiated using 500 mg friable callus in 50 ml MS liquid medium fortified with PGRs viz. 2,4-D (2 mg/l) + BAP (1mg/l) in 250 ml of Erlenmeyer flasks. The presence of Gymnemic acid was detected using HPTLC. Cell suspension cultures treated with 0.6 g/l of yeast extract resulted in the maximum production of Gymnemic acid as compared to other tested concentrations of Yeast extract, Pectin and control. The data obtained from the present study could be used for the enhancement of in vitro biosynthesis of commercially significant Gymnemic acid using cell suspension culture. In addition, it will help in reducing uncontrolled overexploitation of Gymnema sylvestre R. Br., which is declared as vulnerable plant in red data book.