Treatment of snakebites envenomings is critically dependent on the availability of effective anti-venoms. Medicinal plants have been extensively studied in order to obtain an alternative treatment. The present in vitro neutralization study aims to provide a scientific explanation for the use of aqueous leaves extraction of S.leucantha against snakebite activity on toxic venom enzymes like hyaluronidase, acetylcholinesterase, 5’nucleotidase and phosphodiesterase, from Indian cobra (Naja naja) venom. Maximum inhibition of Hyaluronidase 72.91%, Acetylcholinesterase 71.76%, 5’nucleotidase 76.48% and Phosphodiesterase 71.16% observed at 100µg/ml concentrations. This observations proved that the aqueous extract has the anti venom efficacy against venom enzymes.