Phymatosorus scolopendria (Burm. F.) Pic. Serm. Is a medicinal plant belonging to the family polypodiaceae. The aim of this study is to evaluate Brine shrimp lethality against whole plant methanolic extract of Phymatosorus scolopendria (Burm. F.) Pic. Serm. To find out its toxicity. The brine shrimp lethality test is an excellent predictive tool for the toxic potential of plant extracts. The study confirms the moderate potent cytotoxic activities of Phymatosorus scolopendria whole plant methanolic extract with LD50 value of 434.27 µg/ml. and therefore demands the isolation of active principles and thorough preclinical and clinical studies.