In this study, Random Amplified Polymorphic DNA (RAPD) marker will be used to study the molecular diversity of 10 popular potato varieties of India. DNA was extracted from tender leaf samples and it was quantified by using UV - Vis spectrophotometer. Total thirteen RAPD primers were used to run test the polymorphism and data was analyzed by DARwin6 software and dendrogram was developed to establish evolutionary relationships among potato varieties. The Polymorphic Information Content (PIC) among varieties ranged between 0.19 and 0.84. All of the RAPD primers had percent polymorphism more than 60 %.