Aspergillus flavus is the main producer of the well-known carcinogenic aflatoxins. The presence of this fungus and aflatoxins is of huge concern in terms of food safety. A.flavus isolated by serial dilution and spread plate techniques. 0.1 ml of 10-3- 10-5 dilution sample spread through over the on Potato Dextrose Agar (PDA) plates. The plates were incubated at 28°C for one week, then the fungal colonies were observed and pure cultures were maintained. The identification of fungi at the genus level was carried out by using macroscopic and microscopic examinations depending on the colony color, shape, hyphae, conidia, conidiophores and arrangement of spores. For the molecular identification of the isolated fungi at the species level, the extracted fungal DNA was amplified by PCR using specific internal transcribed spacer primer (ITS1/ITS4). The PCR products were sequenced and compared with the other related sequences in Gen-Bank (NCBI). Fungal species were identified as: Aspergillus flavus.