As resistance to conventional drugs continues to increase, the growing knowledge on the medicinal values of herbs and herbal products and their subsequent use have endeared researchers to intensified study of medicinal plants. The estimated composition of Rauwolfia vomitoria extracts and its traditional use in the treatment of many infections necessitates the evaluation of its actual composition and effectiveness towards the management of multidrug resistant infections. In this study, bacteria were isolated from urine samples collected from patients in University of Nigeria Medical Centre and Chinemerem Specialist Hospital in Nsukka metropolis. Methanol, chloroform and aqueous extractions of Rauwolfia vomitoria leaves were done. The methanol extract was further fractionated into n-hexane, ethyl acetate and aqueous partitions. Antibiotics sensitivity was done using agar disc diffusion method and Multiple Antibiotics Resistance (MAR) index calculated. Antimicrobial testing of the plant extracts was done using agar well diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined. The resistance induction potential of the plant extract was carried out. The MAR indices ranged from 0.25 to 0.5. Some of the resistant isolates were susceptible to the plant extracts though the MIC (25-50mg/ml) and MBC (200mg/ml) were at high concentrations. This study provides evidence that extracts of R. vomitoria have moderate antibacterial effect against MDR E. coli and K. pneumoniae.