Euphorbia hirta powdered Medicinal plant material was extracted using 4 solvents DMSO, Methanol, Acetone, and Aqueous. The antibacterial activity of the plant leaf extract, the Disc diffusion method, and the Well diffusion method was used to assess the antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumonia and Staphylococcus aureus, human pathogens. Minimum inhibitory concentration (MIC) of DMSO extracts of leaves showed the lowest MIC against four tested microorganisms followed by methanol, acetone, and aqueous extracts. The lowest minimum inhibitory concentration (0.125mg/ml). Minimum bacterial concentration (MBC) of DMSO extracts of leaves showed the lowest MBC against all the tested microorganisms followed by methanol, acetone, and aqueous extracts. The lowest minimum bacterial concentration (0.25mg/ml) of Euphorbia hirta leaves.