Rice disease bacterial leaf blight (BLB) is one of the most destructive diseases throughout the world. The present work is focused on plant growth promoting fungi (PGPF) Trichoderma harzianum(PGT4), Trichoderma reesei(PGT5), and Trichoderma reesei(PGT13), and co-culture of Trichoderma spp.(PGTA). Molecular docking studies were carried out to support the antibacterial activity of PGTA against Xanthomonas oryzae pv. oryzae(Xoo). Docking implies a strong binding affinity of ligand 8 and ligand 10 against both the target proteins suggest that both ligands can block the activity of proteins. Overall, Trichoderma spp. can be used as biocontrol and plant growth promotion in field conditions.