A study of seasonal dynamics of zooplankton diversity with reference to physico-chemical conditions (water quality) of three water bodies (Hiratalai, Swaroop Sagar and RCA nursery pond) in Udaipur, Rajasthan was made in winter (January), summer (May) and late monsoon (September) to determine their ecological health. Zooplankton as a cosmopolitan community is vital ecological indicator for assessment of water quality and biodiversity, as they are strongly affected by environmental conditions and respond quickly to changes in water quality. Zooplankton constitutes a vital intermediate link between phytoplankton and fish in the food chain. Seasonal changes in zooplankton species are related to the physico-chemical parameters of aquatic ecosystem. These parameter analysis helps to evaluate the impact of anthropogenic activities on water bodies. As per the approved technical programme, studies were conducted using the laboratory facilities of limnology and fisheries department of MPUAT, Udaipur and Zoology Department of MLSU, Udaipur. While the field studies were conducted at three different seasonal ponds of Udaipur district.