This study aims to determine the effect of 2,4 Dimethyl amine 865 g/l herbicide on weeds in lowland rice cultivation. Testing was conducted in Baleendah Subdistrict, Bandung Regency, West Java from February 2020 to June 2020. The test design used was a randomized block design with 4 replications and 7 treatments. The treatments consisted of a herbicide dose of 2,4-D amine 865 g/l, namely a dose of 432.5 g/ha; 648.75 g/ha; 865 g/ha; 1081.25 g/ha; 1297.5 g/ha, manual weeding and control treatment (without weed control). The results showed that 2,4 Dimethyl amine 865 g/l herbicide dose 432.5-1297.5 g/ha could control weeds in rice cultivation, including Ludwigia octovalvis, Marsilea crenata, Monochoria vaginalis, Cyperus difformis, weeds others, and total weeds for up to 6 weeks after application. 2,4 Dimethyl amine 865 g/l herbicide dose 432.5-1297.5 g/ha did not cause phytotoxicity to rice plants. 2,4 Dimethyl amine 865 g/l herbicide dose 432.5-1297.5 g/ha can replace manual weeding and provide growth and yield of rice that is not different from manual weeding.