Chromium (Cr) is considered to be a non-essential heavy metal which has a revealing impact on plant. The present study assessed the possible impacts of hexavalent chromium (Cr+6) toxicity on rate of germination, photosynthetic activity, protein, proline, starch and total sugar content of the test plants. The study has been carried out using three different plant species including a nitrogen fixer plant [Sesbania cannabina (Retz.) Pers.], two oil yielding plants [Sesamum orientale L.; Ricinus communis L.] in the liquid culture medium containing different levels of hexavalent chromium. The experiment revealed that at an interval of 21 days with increased Cr (VI) concentrations in the medium showed decreased germination rate, total chlorophyll, protein, starch and total sugar content but there was increase in proline content of the three test plants. It has been concluded that the species Sesbania cannabina (Retz.) Pers. as compared to the other two species i.e. Sesamum orientale L. and Ricinus communis L. observed to exhibit higher resistance to hexavalent chromium toxicity.