In this present study Aegle marmelos L. was taken to investigate its potential for synthesizing silver nanoparticles. The silver nanoparticles synthesized from the ethanolic extract of leaves were confirmed by their change of colour to dark brown due to the phenomenon of surface plasmon resonance. The characterization of nanoparticle synthesized was done by UV-vis spectroscopy, Fourier Transmission infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Dynamic light scattering (DLS) and X-Ray diffraction (XRD) studies. The medicinal plant mediated silver nanoparticles show good antimicrobial activity against clinically important pathogens Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Streptococcus pyogenes.