The plant Coldenia procumbens Linn. is used as a common medicine in Indian system for various illness. The investigation of functional group of phytocompounds by FTIR and GC-MS analysis from methanolic extracts of the Coldenia procumbens. The FTIR spectra revealed the occurrence of functional characteristic peaks of aromatic amines, carboxylic acids, ketones, phenols and alkyl halides group, etc. from aerial parts of Coldenia procumbens extracts. The GC-MS analysis of methanol extracts from aerial parts of Coldenia procumbens detected the presence of 58 phytochemical compounds. The biological significance of the extract of antimicrobial, cytotoxicity, anti-inflammatory, pharmacological and hepatoprotective activity etc. were studied. The detailed results were recorded and discussed.