Antifungal activity of crude methanolic and ethanolic leaves and stem extracts of Ocimum sanctum was evaluated to calculate zone of inhibition. The ethanolic extract of Ocimum sanctum showed more antifungal activity in relative to methanol extracts against two clinical fungus Aspergillus flavus and candida albicans. It is clear from the current study that the Ocimum sanctum represents high antifungal activity against A. flavus in methanolic stem and ethanolic leaf extracts while methanolic leaf and ethanolic stem extract have high antifungal activity against C. albicans. We conclude in this study that Ocimum sanctum has high antifungal activity against both fungus and more antioxidant activity because of the presence of flavonoid content.