The main source of fungal microflora is originate from materials of plant origin, primarily cereals and feed samples. In relation to humans and animals, mycotoxins exhibit toxic action and are characterized by carcinogenic, mutagenic, teratogenic and estrogenic properties. Due to the diversity of toxic effects, as well as their resistance to the action of high tempareture, the presence of mycotoxins in feeds constitutes a potential threat to human and animal health. Incidence of diffent species of Aspergillus were analyzed and isolated from 87 poultry feed samples (starter, breeder, boiler and layer) collected from different places of Telangana region. In all samples, 357 strains representing elevan species of Aspergillus such as A. flavus, A. flavipus, A. fumigatus, A. glucus, A. niger, A. nidulans, A. ochreceus, A. parasiticus, A. terreus, A. ustus and A. versicolor were probably recorded. All the feed samples were contaminated with a number of different fungal species. These 357 strains of Aspergillus were screened for mycotoxin production by Thin Layer Chromatography (TLC) using different spray reagents. Among them, 165 strains representing 11 species were recorded to be positive for production of aflatoxin, oxalic acid, ochratoxin A, patulin, aflatrem, gliotoxin, nidulotoxin, citrinin and sterigmatocystin etc.